BCR/ABL hybrid gene detection – semi-quantitative analysis


The t(9;22) translocation occurs in the majority of patients suffering from chronic myeloid leukemia (CML) but also in a relatively large percentage of patients with acute lymphocytic leukemia (ALL), leading to the expression of a hybrid RNA of the BCR/ABL type.

A semi-quantitative RT-PCR method is applied, from a peripheral blood or bone marrow sample, allowing the detection of at least 10 RNA copies of the M-, m-, or μ- BCR/ABL type.

This test may also be applied in combination with: